Fig. 1.

MiR-10a is differentially expressed in the aMPN, CML, RHL and HD groups. a Blood leukocyte RNA from patients with aMPN (n = 18) or CML (n = 10) were analyzed on an Human miRNA Microarray V2 (Agilent Technologies). The volcano plot represents the analysis of differentially expressed miRNAs in aMPN as compared to CML: the expression difference in miRNA expression is plotted on the x axis, and p-value significance is plotted on the y axis (−log10 scale). MicroRNAs that are found differentially expressed (p < 0.001) are arrow-pointed. b qRT-PCR quantification of miR-10a in peripheral blood cells from healthy donors (HD, n = 7), donors with reactive hyperleukocytosis (RHL, n = 14), patients with CML at diagnosis (n = 10) and patients with aMPN at diagnosis (n = 18) patients. The graph shows relative expression of gene by disease with mean ± SEM