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. 2018 Nov 12;13:3. doi: 10.1186/s13029-018-0070-0

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Informatics workflow for shotgun proteomics studies: a Liquid biopsies taken at time of surgery. b Liquid biopsies are processed for proteomic analysis. c Liquid chromatography-tandem mass-spectrometry used to analyze protein content. d Protein IDs are matched to peptide mass-spectral data. e Protein IDs and mass-spectra data are organized. f Samples (control vs. disease, etc.) are compared based on protein contents. g Quantitative data is lost during comparative analysis. h ProSave inputs original data and bare protein IDs, then outputs (i) restored protein-data pairs for trend analysis