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. 2018 Nov 12;37:273. doi: 10.1186/s13046-018-0921-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — DANCR positively regulates MSI2 expression via sponging miR-149. a: The RNA-FISH results revealed that DANCR was distributed mostly in the cytoplasm in bladder cancer cells. b: The comprehensive transcriptional analysis results showed that DANCR expression levels were statistically positively correlated with MSI2 expression levels in bladder cancer. c: Knockdown of DANCR decreased MSI2 expression in bladder cancer cells. d: The bio-information analysis results showed that DANCR and MSI2 have common putative binding sites with multiple miRNAs. e: Knockdown of DANCR increased miR-149 expression in bladder cancer cells. f: Bioinformatics analysis showed that the 3’UTR sequence of MSI2 is complementary to the seed sequence of miR-149. Dual-luciferase reporter assay showed MSI2-Wt and Agomir149 co-transfection significantly inhibited luciferase activity. g: Knockdown of DANCR decreased the luciferase activity of bladder cancer cells transfected with MSI2-Wt. h: Overexpressing miR-149 decreased the expression of MSI2 in bladder cancer cells. i: Knockdown of miR-149 increased MSI2 expression in bladder cancer cells transfected with shRNA-DANCR. Data are shown as mean ± SD. *p < 0.05; **p < 0.01