Table 2.
Cold acclimation duration (days) | Helin (Norway) | Leitrim (Ireland) | |
---|---|---|---|
% survival after exposure to − 9 °C | 0 | 0 | 8 |
2 | 27 | 46 | |
14 | 81 | 76 | |
Fv/Fm after exposure to − 9 °C | 2 | 0.35 (± 0.06) | 0.57 (± 0.04) ** |
14 | 0.43 (± 0.02) | 0.48 (± 0.03) ns | |
Electrolyte leakage (%) at −9 °C | 0 | 96.0 (± 2.0) | 97.3 (± 0.2) ns |
2 | 99.3 (± 1.0) | 89.2 (± 2.6) * | |
14 | 48.2 (± 13.0) | 74.8 (± 17.0) ns | |
TEL50 (°C) | 0 | −4.2 | −4.5 |
2 | −6.7 | −7.6 | |
14 | −9.2 | −7.8 |
Sub-zero temperatures were applied at a rate of − 2 °C per hour after 0, 2 or 14 days cold acclimation at 2 °C. Statistically significant differences between populations are indicated by * = P ≤ 0.05; ** = P ≤ 0.01; ns = not significant