Table 2:
Activity of GphF DH1/L1744P
| Percent distribution of substrate & products (cmpd) | |||
|---|---|---|---|
| Starting material | 3-hydroxy | 2-enoyl | 3-enoyl |
| 2 | 51.3 ± 0.4 (2) | 48.7 ± 0.4 (6) | < 0.01 (14) |
| 3 | 50.3 ± 0.6 (3) | 49.6 ± 0.5 (6) | < 0.01 (14) |
| 4 | 99.3 ± 0.1 (4) | 0.6 ± 0.1 (6) | < 0.01 (14) |
| 5 | 99.9 ± 0.1 (5) | < 0.01 (6) | < 0.01 (14) |
| 6 | 12.9 ± 0.2 (2) | 87.1 ± 0.2 (6) | < 0.01 (14) |
| rac-7 | < 0.01 (2) | < 0.01 (6) | 99.9 ± 0.1 (rac-7) |
| 8 | < 0.01 (2) | < 0.01 (6) | 99.9 ± 0.1 (8) |
| 9 | 58.9 ± 0.1 (9) | 39.1 ± 0.5 (10) | 2.0± 0.1 (11) |
| 10 & 11 (2:1) | 35.1 ± 0.2 (9) | 35.8 ± 0.8 (10) | 29.1 ± 0.5 (11) |
| 12 | 4.3 ± 0.1 (2) | 95.6 ± 0.1 (6 & 12) | < 0.01 (14) |
| 13 | 39.7 ± 0.6 (9) | 40.7 ± 0.5 (10 & 13) | 19.6 ± 0.2 (11) |