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. Author manuscript; available in PMC: 2019 Sep 21.
Published in final edited form as: ACS Chem Biol. 2018 Sep 12;13(9):2699–2707. doi: 10.1021/acschembio.8b00645

Table 3:

Activity of GphF DH1/Y1856F

Percent distribution of substrate & products (cmpd)
Starting material 3-hydroxy 2-enoyl 3-enoyl
2 83.7 ± 0.6 (2) 16.2 ± 0.7 (6) < 0.01 (14)
3 80.5 ± 0.4 (3) 19.5 ± 0.4 (6) < 0.01 (14)
4 99.4 ± 0.1 (4) 0.6 ± 0.1 (6) < 0.01 (14)
5 99.9 ± 0.1 (5) < 0.01 (6) < 0.01 (14)
6 2.3 ± 0.3 (2) 97.7 ± 0.3 (6) < 0.01 (14)
rac-7 < 0.01 (2) < 0.01 (6) 99.9 ± 0.1 (rac-7)
8 < 0.01 (2) < 0.01 (6) 99.9 ± 0.1 (8)
9 61.4 ± 0.6 (9) 31.9 ± 0.5 (10) 6.6 ± 0.3 (11)
10 & 11 (2:1) 31.1 ± 0.3 (9) 38.5 ± 0.4 (10) 30.4 ± 0.2 (11)
12 0.6 ± 0.1 (2) 99.3 ± 0.1 (6 & 12) < 0.01 (14)
13 33.8 ± 0.1 (9) 39.9 ± 0.2 (10 & 13) 26.3 ± 0.2 (11)