Table 3:
Activity of GphF DH1/Y1856F
| Percent distribution of substrate & products (cmpd) | |||
|---|---|---|---|
| Starting material | 3-hydroxy | 2-enoyl | 3-enoyl |
| 2 | 83.7 ± 0.6 (2) | 16.2 ± 0.7 (6) | < 0.01 (14) |
| 3 | 80.5 ± 0.4 (3) | 19.5 ± 0.4 (6) | < 0.01 (14) |
| 4 | 99.4 ± 0.1 (4) | 0.6 ± 0.1 (6) | < 0.01 (14) |
| 5 | 99.9 ± 0.1 (5) | < 0.01 (6) | < 0.01 (14) |
| 6 | 2.3 ± 0.3 (2) | 97.7 ± 0.3 (6) | < 0.01 (14) |
| rac-7 | < 0.01 (2) | < 0.01 (6) | 99.9 ± 0.1 (rac-7) |
| 8 | < 0.01 (2) | < 0.01 (6) | 99.9 ± 0.1 (8) |
| 9 | 61.4 ± 0.6 (9) | 31.9 ± 0.5 (10) | 6.6 ± 0.3 (11) |
| 10 & 11 (2:1) | 31.1 ± 0.3 (9) | 38.5 ± 0.4 (10) | 30.4 ± 0.2 (11) |
| 12 | 0.6 ± 0.1 (2) | 99.3 ± 0.1 (6 & 12) | < 0.01 (14) |
| 13 | 33.8 ± 0.1 (9) | 39.9 ± 0.2 (10 & 13) | 26.3 ± 0.2 (11) |