Table 4.
CurK DH Activity
| Percent distribution of substrate & products (cmpd) | |||
|---|---|---|---|
| Starting material | 3-hydroxy | 2-enoyl | 3-enoyl |
| Wild type | |||
| 9 | 49.1 ± 0.2 (9) | 50.9 ± 0.2 (10) | < 0.01 (11) |
| 10 & 11 (2:1) | 34.8 ± 0.3 (9) | 34.9 ± 0.1 (10) | 30.3 ± 0.3 (11) |
| P1005V | |||
| 9 | 60.2 ± 0.3 (9) | 38.4 ± 0.2 (10) | 1.4 ± 0.2 (11) |
| 10 & 11 (2:1) | 34.9 ± 0.3 (9) | 36.9 ± 0.5 (10) | 28.2 ± 0.7 (11) |