Figure 3: Daxx heterozygosity contributes to carcinoma development in cooperation with low dose ionizing radiation.

A) Schematic representation of experimental procedure. Mice were treated with four weekly doses of ionizing radiation (IR, 4 × 0.7 Gy), starting at 4 weeks of age. Mice were monitored until moribund, or until the 2-year experimental endpoint. B) Survival analysis. C) Tumor spectrum of mice from both genotypes. Chi-squared analysis of the proportions of different tumor types in Daxx^Δ3/+ compared to those expected from wild-type controls, *p<0.013. Tumors that were present when mice were moribund or autopsied are included in this analysis. D) Representative H&E sections of identified ovarian pathology from both genotypes. Cysts were identified only in Daxx^Δ3/+ mice, and carcinomas in both genotypes. Cyst is at 10x and carcinomas at 40x magnification. E) Representative H&E sections of papillary carcinomas observed in Daxx^Δ3/+ mice. Images are at 20x magnification. F) Immunoblot analysis of four Daxx^Δ3/+ tumors. HCC, hepatocellular carcinoma; Vcl, vinculin. G) H&E sections of islet pathology identified in Daxx^Δ3/+ mice. Images are at 10x magnification.