Figure 2. Day/night physical activity in naive mice and mice with skeletal pain with and without anti-NGF.

In naive mice (black circles) and mice with fracture + vehicle (upside down red triangles) or fracture + anti-NGF (green triangles) the horizontal and vertical activity and average velocity (A-C respectively) of the mice was continuously recorded for 20 hours, from 1200 (noon) to 0800 hours the following morning under a 12-hour light/dark cycle (0700–1900 hours light and 1900–0700 hours dark). Note that the greatest level of activity and the greatest differences between the three groups was during the first hour the mice were placed in the activity box (1200 to 1300 hours) and the first 3 hours of dark (1900–2100).