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. 2018 Aug 29;154(10):1191–1198. doi: 10.1001/jamadermatol.2018.2756

Figure 5. Methotrexate-Induced Increase in Gentian Violet (GV)–Induced Apoptosis.

Figure 5.

Cells were treated with GV (1 μmol/L), methotrexate (10 μmol/L), methotrexate and GV, or dimethyl sulfoxide (DMSO) (1 μmol/L) for 24 hours; apoptosis was detected by flow cytometry. There was no significant difference (P > .05) between methotrexate and GV vs GV treatment in SeAx cells. The Jurkat T-cell acute lymphoblastic luekemia line was included for comparison.

aP < .001 for methotrexate and GV treatment vs DMSO, methotrexate, and GV.

bP < .001 for methotrexate and GV treatment vs DMSO and methotrexate.