Table 4. Analysis of MYD88 L265P in Samples of Patients With Vitreoretinal Lymphoma Compared With Routine Laboratory Tests in Diagnostic Workup of Vitreoretinal Lymphoma.
| Variable | Samples, No. (%) | |||
|---|---|---|---|---|
| Patients With Vitreoretinal Lymphoma | Patients With Uveitis | |||
| Positive for MYD88 L265P | Negative for MYD88 L265P | Positive for MYD88 L265P | Negative for MYD88 L265P | |
| Vitreous fluid | ||||
| Cytomorphologya | ||||
| Positive for MYD88 L265P | 5 (83) | 1 (17) | 0 | 0 |
| Negative for MYD88 L265P | 10 (71) | 4 (29) | 0 | 9 (100) |
| Flow cytometrya | ||||
| Positive for MYD88 L265P | 7 (70) | 3 (30) | 0 | 0 |
| Negative for MYD88 L265P | 5 (71) | 2 (29) | 0 | 12 (100) |
| Aqueous humorb | ||||
| Cytomorphologya | ||||
| Positive for MYD88 L265P | 3 (75) | 1 (25) | 0 | 0 |
| Negative for MYD88 L265P | 4 (50) | 4 (50) | 0 | 1 (100) |
| Flow cytometrya | ||||
| Positive for MYD88 L265P | 5 (63) | 3 (38) | 0 | 0 |
| Negative for MYD88 L265P | 4 (80) | 1 (20) | 0 | 2 (100) |
Abbreviations: ddPCR, droplet digital polymerase chain reaction; MYD88, myeloid differentiation primary response gene 88.
Samples of vitreous fluid used for cytomorphology came from 20 patients, vitreous fluid samples for flow cytometry were from 18 patients, aqueous humor samples for cytomorphology were from 18 patients, and aqueous humor samples for flow cytometry were from 18 patients. In several cases, multiple samples were used from single patients.
Since cytomorphology and flow cytometry are usually not performed on aqueous humor samples, the performance of MYD88 analysis in aqueous humor was compared with the simultaneous evaluation of cytomorphology and flow cytrometry in vitreous fluid. An overview of test results per patient are outlined in eTable 5 in the Supplement.