Table 1.
Migration analysis of MDA-MB-231 cells after the treatment with 5-LOX inhibitors
Treatment | MDA-MB-231, 0 h | MDA-MB-231, 24 h |
---|---|---|
Mean ± SD | Mean ±SD | |
Control | 2.3 ± 1.3 | 79 ± 7.6 |
AA | 3.4 ± 2.2 | 119.7 ±18.9 |
NDGA | 2±1.4 | 9 ± 1.7 |
AA + NDGA | 4±1.5 | 84.7±18.9 |
Zil | 2.2 ± 1.1 | 40 ± 5.2 |
AA + Zil | 2.3 ± 1 | 69 ± 10 |
AA + LTB4 | 1.6 ±1.2 | 138.3 ± 4.7 |
LTB4 + NDGA | 1 ± 1.3 | 48.3 ± 1.7 |
AA + NDGA+ LTB4 | 2.3 ± 2.2 | 67.3 ±10.8 |
LTB4 + Zil | 0.3 ±1.1 | 62.7± 5.8 |
AA + Zil + LTB4 | 2.6 ±1.5 | 68 ±12.4 |
AA + PGD2 | 4.3±1 | 62 ± 6.2 |
PGD2 + NDGA | 2.3±1.5 | 44.3± 5.2 |
AA + NDGA+ PGD2 | 1.5 ±2.6 | 58.7 ±18.1 |
PGD2 + Zil | 1.6±2.3 | 61.6 ±15.7 |
AA + Zil + PGD2 | 3.6 ± 1.2 | 43 ±14.8 |
Results shown here are the average values (± SD) obtained from migration assays after various treatments. The experiments were repeated twice in triplicate on two different days. SD, Standard deviation; LTB4, leukotriene B4; NDGA, nordihydroguaiaretic acid; PGD2, prostaglandin D2.