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. 2018 Nov 13;16(11):e2006522. doi: 10.1371/journal.pbio.2006522

Fig 1. H3K36me3 changed accordingly with SETD2 level during BMSC adipogenesis and osteogenesis.

Fig 1

(A) qPCR analysis of Setd2 and adipocyte markers including Pparγ1, Pparγ2, Cebpα, Fabp4, and Perilipin expression in BMSCs during adipogenesis for indicated days. Data represent mean ± SD, n = 4. (B) Western blot analysis of SETD2, PERILIPIN, and H3K36me1/2/3 level during adipogenesis for different days. (C) qPCR analysis of Setd2 and osteoblast markers including Runx2, Osx, Col1α1, Alp, and Bsp expression during osteogenesis for different days. Data represent mean ± SD, n = 4. (D) Western blot analysis of SETD2, OSX, and H3K36me1/2/3 level in BMSCs during osteogenesis for indicated days. (E) Western blot analysis of SETD2 and H3K36me1/2/3 level in BMSCs isolated from WT and Prx1-Cre, Setd2fl/fl mice. (F) Immunohistochemistry assay of SETD2 and H3K36me3 level in hindlimb growth plate of Prx1-Cre, Setd2fl/fl mice and WT control mice. Images are representative results from 3 independent experiments. Scale bar = 20 μm. Data used in the generation of this figure can be found in S1 Data. Alp, alkaline phosphatase; BMSC, bone marrow mesenchymal stem cell; Bsp, bone sialoprotein; Cebpα, CCAAT enhancer binding protein α; Col1α1, Collagen type 1 alpha 1; Fabp4, fatty acid binding protein 4; H3K36me3, H3 lysine 36 trimethylation; Osx, Sp7 transcription factor; Pparγ1/2, peroxisome proliferative activated receptor γ1/2; qPCR, quantitative PCR; Runx2, runt-related transcription factor 2; SETD2, SET-domain-containing 2; WT, wild-type.