Skip to main content
. 2018 Nov 1;14(11):e1007389. doi: 10.1371/journal.ppat.1007389

Fig 5. Loss of PAN RNA does not affect the activity of the KSHV late lytic transcription factor complex.

Fig 5

BCBL-1 cells were electroporated with oligonucleotides antisense either to GFP mRNA (control KD) or to KSHV PAN RNA and then induced with 600 μM valproic acid for 48 h. RNA was harvested and analyzed by qPCR to determine changes in viral transcription factor mRNA levels (A) and expression of target genes of the viral late lytic transcription factor complex (B). The approximate change in mRNA levels upon knockout of the viral transcription factor ORF31 are shown as a dotted line for reference [33]. Data are the average of three biological replicates. (C) HEK293T cells were transiently transfected with viral late lytic transcription factor expression plasmids as well as luciferase reporter plasmids (pGL4). The firefly luciferase ORF was located downstream of the viral ORF57 promoter (pGL4 ORF57) or the viral K8.1 promoter (pGL4 ORFK8.1). vPIC-ORF24 is a negative control sample that lacks the ORF24 expression plasmid. RTA is a transcription factor that is required for expression of PAN RNA. Samples were normalized to pGL4 ORFK8.1+vPIC+vector. Data represent the average of three biological replicates, each done in three technical replicates.