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. 2018 Nov 1;14(11):e1007777. doi: 10.1371/journal.pgen.1007777

Fig 4. Antibiotic treatment partially rescues the decrease in lifespan but not enterocyte cell death due to loss of dMyc.

Fig 4

(A) Diptericin mRNA expression in dissected fat bodies of 21 day old 5966-GS>dMyc RNAi flies with/without antibiotic treatment. (B, C and D) puc mRNA (B), hid mRNA (C) and upd3 mRNA (D) expressions in dissected guts of 21 day old 5966-GS>dMyc RNAi flies with/without antibiotic treatment. (E) Mitotic ISCs quantification in 21 day old 5966-GS>dMyc RNAi flies with/without antibiotic treatment. Error bars indicate SEM of 13 guts. (F) Smurf gut permeability assay in 20 day old 5966-GS>dMyc RNAi flies with/without antibiotic treatment. Control (–RU486) without antibiotic (AL: n = 185), 5966-GS>dMyc RNAi (+RU486) without antibiotic (AL: n = 222, DR: n = 199), Control (–RU486) with antibiotic (AL: n = 233), 5966-GS>dMyc RNAi (+RU486) with antibiotic (AL: n = 173, DR: n = 238) Error bars indicate SEM of 12 different vials. (G) Kaplan-Meier survival analysis of 5966-GS>dMyc RNAi flies with/without antibiotic treatment upon AL and DR. (A-D) Error bars indicate SD from 3 independent biological replicates. ‘AB’ represents antibiotic. Statistical analysis of the survival curves and the number of flies are provided in S2 and S3 Tables.