Table 2 :
Interactions measured by microcalorimetry between Ku70/Ku80, A-KBM, X-KBM and XLF
| Protein in the measurement cell | Ligand in the syringe | Kd (μM) | ΔH (kcal.M−1) | Remarks | |
|---|---|---|---|---|---|
| 1 | KuFL | pAPLF | 0.033 ± 0.01 | −13.3 ± 0.1 | A-KBM (APLF 174–191) |
| 2 | KuFL | DNA 18bp | 0.0041 ± 0.0007 | +5.3 ± 0.2 | DNA18bp versus Ku |
| 3 | KuFL+DNA-18bp | pAPLF | 0.023 ± 0.002 | −16.0 ± 0.4 | in presence of DNA18bp |
| 4 | KuCC | pAPLF | 0.020 ± 0.002 | −18.4 ± 0.7 | Ku without Cter domains |
| 5 | KuFL I112R | pAPLF | NI(a) | NI | Ku80 mutant on APLF site |
| 6 | KuFL | pXLF | 4.4 ± 0.2 | −2.8 ± 0.2 | X-KBM (XLF 281–299) |
| 7 | KuFL +DNA-18bp | pXLF | 2.4 ± 0.1 | −8.1 ± 0.6 | in presence of DNA18bp |
| 8 | KuCC | pXLF | 2.2 ± 0.9 | −3.1 ± 1.2 | Ku without Cter domains |
| 9 | KuFL | XLF | 1.0 ± 0.1 | −9.0(b) | XLF versus Ku |
| 10 | KuFL+DNA-18bp | XLF | 2.35 ± 0.1 | −8.1 ± 0.6 | XLF versus Ku/DNA18bp |
| 11 | KuFL | pXLF(LW) | 0.12 ± 0.03 | −12.1 ± 2.0 | X-KBM (L297W) |
| 12 | KuFL | pXLF(LE) | NI | NI | X-KBM (L297E) |
| 13 | KuFL+pAPLF | pXLF(LW) | NI | NI | L297W in presence of A-KBM |
(a)
NI means no interaction;
(b)
The enthalpy value was deduced from the first injection point in absence of lower plateau; The thermograms and isotherms of titration of the experiments corresponding to lines 1, 6 and 11 are presented in Supplementary Figure 2e–g. Interactions in lines 1, 4, 6, 8, 9, 11 were measured in triplicate, and the mean value with standard deviation is reported. Interactions in lines 2, 3, 4, 6, 10 were measured in duplicate, and the mean value with variation between min and max values is reported.