Figure 1.

Activation of kinases and their role in Panc1 cell invasion. Panc1 cells were treated with 200 μM omeprazole (A) and 15 nM TCDD (B) for up to 12 hr, and whole cell lysates were analyzed by western blots as outlined in the Materials and Methods. Role of AhR in mediating effects of omeprazole (C). Cells were transfected with a non-specific oligonucleotide (−) or siAhR (+), treated with 200 μM omeprazole for up to 6 hr, and whole cell lysates were analyzed by western blots. Effects of kinase inhibitors on invasion. Panc1 cells were treated with 200 μM omeprazole in the presence or absence of several kinase inhibitors, and effects on cell invasion were determined in a Boyden chamber assay (D) and quantitated (E). (F) Panc1 cell spheroids were treated with DMSO or omeprazole, and spheroid growth was determined as outlined in the Materials and Methods. Results are expressed as means ± SD for at least 3 replicates per treatment group, and significant (p<0.05) inhibition of invasion (*) and reversal of these effects by kinase inhibitors are indicated (**).