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. 2018 Nov 13;7:e38865. doi: 10.7554/eLife.38865

Figure 4. Treatment with a CSF-1R inhibitor alters myeloid cell composition in the contralateral hemisphere.

(A) Plot of microglia (CD11b + CD45 lo) numbers in treated tumor bearing mice. There was significant CSF-1R inhibitor treatment effect (F(1,8) = 31.45, p=0.0005), but no significant radiation effect or interaction between these two treatments. Two-way ANOVA with Dunnett post-hoc comparisons against the tumor +DT group, ns = not significant, *p<0.05. (B) Plot of inflammatory monocyte (CD11b + CD45hiLy6Chi) numbers in treated tumor bearing mice. There was significant radiation effect (F(1,8) = 10.68, p=0.0114) and CSF-1R inhibitor treatment effect (F(1,8) = 23.49, p=0.0013), as well as significant interaction (F(1,8) = 5.52, p=0.0467) between these two treatments, two-way ANOVA with Dunnett post hoc comparisons against the tumor +DT group, ns = not significant, *p<0.05. Error bars show mean ±SEM values. Each dot represents cell count from a mouse, N = 4–5.

Figure 4—figure supplement 1—source data 1. Source data are uploaded in Figure 4-figure supplement-source data 1.
elife-38865-fig6.xlsx (12.1KB, xlsx)
DOI: 10.7554/eLife.38865.015
Figure 4—source data 1. Source data are uploaded in Figure 4—source data 1.
elife-38865-fig4-data1.xlsx (8.9KB, xlsx)
DOI: 10.7554/eLife.38865.016

Figure 4.

Figure 4—figure supplement 1. Changes in myeloid cells in the ipsilateral hemisphere and growth of tumors with single treatments.

Figure 4—figure supplement 1.

(A) Plot of microglia numbers in tumor bearing mice. (B) Plot of inflammatory monocyte numbers. (C) Plot of BLI signals after single treatments. (D) Plot of correlation between BLI signal (X axis) and monocyte numbers in the ipsilateral brain (Y axis). N = 3–5 each group.