Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus), C57BL/6J |
C57BL/6J | Jackson Laboratory, cat# 000664 |
cat # 000664 | |
| Cell line (Mus musculus), C57BL/6 |
GL261 | NCI-Frederick DCTD tumor/cell line repository |
Glioma 261 | Mycoplasma negative |
| Transfected construct |
ATF5-DTR- GFP |
PMID: 20495567 | NA | |
| Antibody | AF700- CD11b |
BD Biosciences |
cat# 557960 |
Flow Cyt: 1:100 |
| Antibody | BV711- CD45 |
BD Biosciences |
cat# 563709 |
Flow Cyt: 1:100 |
| Antibody | CD68 | Bio-Rad, | cat#: MCA1957 | IF: 1:200 |
| Antibody | Iba1 | Wako chemicals, |
cat# 019–19741 | IF: 1:100 |
| Commercial assay or kit |
Neural Tissue Dissociation kit (P) |
Miltenyi Biotech |
cat# 130-092-628 | |
| Chemical compound, drug |
CSF-1R inhibitor | Plexxikon Inc | PLX5622 | 300ppm in AIN-76A standard chow |
| Other | AIN-76A standard chow |
Research Diets Inc |
cat#: D10001i | |
| Chemical compound, drug |
Percoll | Sigma | cat# P1644-100ML | |
| Chemical compound, drug |
Diphtheria toxin |
Sigma | cat# D0564-1MG | |
| Software | Ethovision XT |
Noldus Information Technology |
Version 11.5 |