Figure 1. (A) The right antennal lobe of a female Drosophila melanogater wholemounted and immunolabelled with nc82 to detect Bruchpilot at synaptic sites, revealing the neuropiles of the brain.

These included the glomeruli of the antennal lobe (AL). Va1v of the right antennal lobe (blue) is enclosed by a box in A, identified in synapse cloud images. Scale bar 100 µm. (B) Image of a single plane with synapses identified as clouds by synapse detection on a corresponding FIB-SEM image stack, enclosing most of the right antennal lobe within which different glomeruli are identified and colour coded. Glomerular borders are visible in this single image from local rarefactions in the density of recognized synapse puncta. (C) A cloud of synaptic puncta in two image planes parallel and corresponding to the one in (B), with different glomeruli – including VA1v (left panel) – identified.