Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (mus musculus, C57BL/6J) |
The Jackson Laboratory |
Stock No: 000664 (Black 6) RRID:IMSR_JAX:000664 |
||
| Strain, strain background (mus musculus, C57BL/SJ) |
Bid-/- | PMID: 10476969 | ||
| Cell line (mouse) | Myeloid Progenitor Cells (MPCs) |
PMID: 16122425 | ||
| Gene (mouse) | BID (BH3 interacting death domain agonist) |
PMID: 8918887 NCBI Reference |
MGI:108093 NM_007544.4 |
|
| Transfected construct |
pOZ-FH-C-hCD25 | PMID: 14712665 | Available from Addgene (plasmid #32516) |
|
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-Bid | PMID: 8918887 | ||
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidBH3 | PMID: 8918887 | ||
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidD59A | PMID: 12519725 | ||
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidBH3AA | This paper | Mutant made with site directed mutagenesis of FHA-BidBH3 construct; Zinkel Laboratory; See Table 1 for primer sequences |
|
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidBH3/M148T | This paper | Mutant made with site directed mutagenesis of FHA-BidBH3 construct; Zinkel Laboratory; See Table 1 for primer sequences |
|
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidM148T | This paper | Mutant made with site directed mutagenesis of FHA-Bid construct; Zinkel Laboratory; SeeTable 1 for primer sequences |
|
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidBH3/E120D | This paper | Mutant made with site directed mutagenesis of FHA-BidBH3 construct; Zinkel Laboratory; See Table 1 for primer sequences |
|
| Transfected construct (pOZ-FH-C-hCD25 vector) |
FHA-BidBH3/R124Q | This paper | Mutant made with site directed mutagenesis of FHA-BidBH3 construct; Zinkel Laboratory; See Table 1 for primer sequences |
|
| Antibody | Bid (goat polyclonal) | R and D systems | AF860 RRID: AB_2065622 |
1:1000 (5% milk, Western Blot (WB)) |
| Antibody | Bid (rabbit polyclonal) | PMID: 8918887 | Antibody generated by S. Korsmeyer Lab |
1:1000 (5% milk, WB) |
| Antibody | Bim (H-5, mouse monoclonal) |
Santa Cruz Biotech-nology |
sc-3743589 RRID: AB_10987853 |
1:100 (5% milk, WB) |
| Antibody | Bad (Clone 48, mouse) | BD Biosciences | 610391 RRID: AB_397774 |
1:500 ((5% milk, WB) |
| Antibody | Puma/bbc3, N-terminal (rabbit) |
Sigma-Aldrich | P4743 RRID: AB_477351 |
1:1000 (5% milk, WB) |
| Antibody | Anti-HA (rabbit polyclonal) |
Sigma-Aldrich | H6908 RRID: AB_260070 |
1:1000 (5% milk, WB) |
| Antibody | VDAC1 (rabbit polyclonal) |
Abcam | ab15895 RRID: AB_2214787 |
1:1000 (5% milk, WB) |
| Antibody | β-Actin (AC-15, mouse monoclonal) |
Sigma-Aldrich | A5441 RRID: AB_476744 |
1:200,000 (5% milk, WB) |
| Antibody | Anti-GAPDH (FL-335, rabbit polyclonal) |
Santa Cruz Biotech-nology |
sc-25778 RRID: AB_10167668 |
1:1000 (5% milk, WB) |
| Antibody | Bak, NT (rabbit polyclonal) |
EMD Millipore | Cat #06–536 RRID: AB_310159 |
1:1000 (5% milk, WB) |
| Antibody | MnSOD (rabbit polyclonal) |
Stressgen | ADI-SOD-111 RRID: AB_10631750 |
1:1000 (5% milk, WB) |
| Antibody | Mcl-1 (rabbit polyclonal) |
Rockland Immunochemi-cals Inc |
600-401-394S RRID: AB_2266446 |
1:1000 (5% milk, WB) |
| Antibody | Opa-1 (Clone 18, mouse) |
BD Biosciences | 612606 RRID: AB_399888 |
1:1000 (5% milk, WB) |
| Antibody | Calreticulin (D3E6, XP, rabbit monoclonal) |
Cell Signaling Technology | 12238 RRID: AB_2688013 |
1:1000 (5% milk, WB) |
| Antibody | Anti-pyruvate dehydrogenase E2/E3 (mouse monoclonal) |
Abcam | ab110333 RRID: AB_10862029 |
1:1000 (5% milk, WB) |
| Antibody | Amersham ECL anti-rabbit IgG, HRP- linked (from donkey) |
GE Healthcare | NA934 RRID: AB_772206 |
1:10,000 (5% milk, WB) |
| Antibody | Goat anti-mouse IgG, HRP-conjugate |
Novex | A16072 RRID:AB_2534745 |
1:10,000 (5% milk, WB) |
| Antibody | Donkey anti-goat IgG HRP | Santa Cruz Biotech-nology |
sc-2020 RRID:AB_631728 |
1:10,000 (5% milk, WB) |
| Chemical compound, drug |
Doxorubicin HCl (Dox) | APP Fresenius Kabi USA, LCC |
NDC 63323-883-05 | |
| Chemical compound, drug |
Epinephrine (Epi) | BPI Labs, LLC | NDC 54288-103-10 | |
| Chemical compound, drug | Fugene 6 Transfection Reagent |
Promega | E2691 | |
| Chemical compound, drug |
Lipofectamine 2000 Transfection Reagent |
ThermoFisher Scientific |
11668027 | |
| Commercial assay or kit |
QuikChange XL Site -Directed Mutagenesis Kit, 10 rxn |
Agilent Technologies | 200521 | |
| Commercial assay or kit |
GeneJET Plasmid Miniprep Kit |
Thermo-Fisher Scientific (Thermo Scientific) |
K0503 | |
| Commercial assay or kit |
GenElute HP Plasmid Maxiprep Kit |
Sigma-Aldrich | NA0310-1KT | |
| Commercial assay or kit |
PureLink HiPure Plasmid Maxiprep Kit |
Thermo-Fisher Scientific (Invitrogen) |
K210006 | |
| Software, algorithm | PrediXcan | PMID: 26258848 and other | https://github.com/hakyimlab/PrediXcan | |
| Software, algorithm | S-PrediXcan | Other | https://github.com/hakyimlab/MetaXcan | |
| Other | CARDIoGRAMplusC4D | Other | www.CARDIOGRAMPLUSC4D.ORG | |
| Other | GTEx Consortium (v6p) | PMID: 29022597 and other | http://www.gtexportal.org |