Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Aspergillus nidulans) |
FGSC A1153 | Nayak et al., 2006 |
yA1, pabaA1; argB2; pyroA4, nkuA::bar |
|
| Strain, strain background (Aspergillus nidulans) |
A1153∆gcnE | Nützmann et al. (2011) |
yA1, pabaA1;
gcnE::argB2; pyroA4, nkuA::bar |
|
| Strain, strain background (Aspergillus nidulans) |
A1153∆basR | This study | yA1, pabaA1; basR::argB2; pyroA4, nkuA::bar | |
| Strain, strain background (Aspergillus nidulans) |
A1153tetOn-basR | This study | yA1, pabaA1; argB2::pabaA1-tetOn-basR; pyroA4, nkuA::bar | |
| Strain, strain background (Aspergillus nidulans) |
A1153∆AN8377 | This study | yA1, pabaA1;AN8377::argB2;pyroA4, nkuA::bar | |
| Strain, strain background (Aspergillus nidulans) |
A1153gcnE-3xflag | Nützmann et al., 2011 |
yA1, pabaA1;
gcnE::gcnEp-gcnE-3x-flag-pabaA1; pyroA4, nku::bar |
|
| Strain, strain background (Aspergillus sydowii) |
CBS 593.65 | Westerdijk Fungal Bio Diversity Institute, The Netherlands |
||
| Strain, strain background (Aspergillus sydowii) |
A. sydowii tetOn-basR | This study | Ectopic integration of pUC18 tetON-A. sydowii basR-hph |
|
| Strain, strain background (Streptomyces rapamycinicus) |
ATCC 29253 | Kumar and Goodfellow, 2008 | ||
| Strain, strain background (Streptomyces lividans) |
TK24 | Cruz-Morales et al., 2013 | ||
| Antibody | ANTIFLAG M2 | Sigma-Aldrich, Taufkirchen, Germany |
F3165-5MG | |
| Antibody | Rabbit polyclonal anti-histone H3 |
Abcam, Cambridge, UK |
ab1791 | |
| Antibody | Rabbit polyclonal histone H3K9ac |
Active Motif, La Hulpe, Belgium | 39137 | |
| Antibody | Rabbit polyclonal anti-acetyl-histone H3 (Lys14) |
Merck Millipore, Darmstadt, Germany |
07 – 353 | |
| Commercial assay or kit |
Universal RNA Purification Kit | Roboklon, Berlin, Germany |
E3598 | |
| Chemical compound, drug |
Digoxigenin-11-dUTP | Jena BioScience, Jena, Germany |
NU-803 | |
| Software, algorithm |
GraphPad Prism 5 | GraphPad Software Inc., La Jolla, USA |
||
| Software, algorithm |
Bioconductor package regioneR | Gel et al. (2016) | ||
| Software, algorithm |
Bioconductor package edgeR |
Robinson and Oshlack (2010) | ||
| Software, algorithm |
MACS, version 2.0.1 |
Zhang et al. (2008) | ||
| Software, algorithm |
MUSCLE | Edgar (2004) | ||
| Software, algorithm |
Integrative Genomics Viewer |
Thorvaldsdóttir et al. (2013) | ||
| Software, algorithm |
MEGA6 | Tamura et al. (2013) | ||
| Software, algorithm |
Shimadzu Class-VP software (version 6.14 SP1) | Shimadzu, Duisburg, Germany |