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. 2018 Nov 13;6(21):e13914. doi: 10.14814/phy2.13914

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Published 2018. This article is a U.S. Government work and is in the public domain in the USA. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Production of TNF α and IL‐10 after chronic and acute LPS treatment. (A) TNF and (B) IL‐10 ELISA analysis of conditioned growth medium. RAW 264.7 cells were either Mock‐treated or treated with LPS (10 ng/mL) for the indicated durations in which either the serum‐free growth medium was not changed after treatment (No Media Δ), or the treated growth medium was replaced with un‐treated, serum‐free growth medium 6 h after initial treatment (Media Δ 6 h). Conditioned growth media was harvested 6, 16, 24, 48, and 72 h after initial treatment. (*P < 0.01; **P < 0.001; ***P < 0.0001, LPS – Media Δ vs. LPS – no Media Δ change; N = 3–5).