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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: ACS Chem Biol. 2018 May 17;13(6):1474–1479. doi: 10.1021/acschembio.8b00262

Figure 4. Selectively labeled mitochondrial transcripts in Mito-GFP-UPRT expressing cells.

Figure 4.

A. Enriched cDNA templates derived from RNA of spatially-unrestricted UPRT vs. Mito-GFP-UPRT expressing cells were assayed by PCR with specific primer pairs for nuclear or mitochondrial transcripts (nc: negative control = DMSO; 5EU = 5’Ethynyl uracil). B Assessment of six mitochondrial and two nuclear transcripts (served as negative controls) from RNA of transiently transfected Mito-GFP-UPRT cells exposed to 5EU in a time course by SYBR Green RT-qPCR analysis (n = two technical duplicates)