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. 2018 Nov 13;9(11):1131. doi: 10.1038/s41419-018-1168-7

Fig. 5. Blockage of autophagy by CQ treatment significantly induces cell apoptosis upon silencing of RPS27L.

Fig. 5

a CQ-siRPS27L combination significantly suppressed growth of MB231 and SK-BR3 cells. Cells were transfected indicated siRNA oligos for 48 h, then left untreated or treated with CQ treatment for indicated time periods, followed by ATPlite assay. Data shown are mean ± SEM. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001. b, c CQ-siRPS27L combinational treatment caused a higher induction of cell apoptosis. MB231 (b) and SK-BR3 cells (b, c) were transfected indicated siRNA oligos for 48 h, and then left untreated or treated with 50 µM CQ for 24 h, followed by IB with indicated Abs (b), or by flow cytometry (c) (left, a representative FACS profile, and right, the percentage of cells at sub-G1 phase, mean ± S.E.M.; n = 3; *p < 0.05.)