FIG 2.

A predicted nucleolar targeting sequence in loop 1 is required for nucleolar localization. (A) Alignment of A3H, A3A, and A3B C-terminal domain amino acid sequences flanking loop 1 (dashed line). The predicted nucleolar localization sequence of A3H is indicated by the solid line. (B) Quantification of nucleolar fluorescence intensity of the indicated A3H constructs (n = 50 nucleoli). The dashed lines highlight ±SEM from wild-type quantification. ns, no significance; ***, P < 0.001 by the unpaired Student t test. (C and D) Representative images of the indicated mCh-A3H constructs coexpressed with FBL-eGFP. ROI are indicated by arrows. Basic-to-Ala/Glu refers to amino acids K16 to R21 replaced by either alanine or glutamate. All scale bars, 5 μm.