FIG 5.
Loop 3 of A3H contributes to nucleolar retention along with the RNA binding interface. (A) Alignment of wild-type A3H, A3A, and an A3AA3H-RNA chimera (A3AA3H-RNA has loop 1, loop 7, and α-helix 6 exchanged from A3H) amino acid sequences that highlight loop 3 (boxed sequence). (B) Structural representation of A3H overlaid with A3A to highlight the proximity of loop 3 to loop 1. (C to E) Representative images of the indicated A3A and A3H constructs. ROI are indicated by arrows. (F) Quantification of nucleolar fluorescence intensity of the indicated A3H constructs (n = 50 nucleoli). The dashed lines highlight ±SEM from wild-type quantification. ns, no significance; ***, P < 0.001 by the unpaired Student t test. All scale bars, 5 μm.