FIG 6.

Free RACK1 represses translation in living cells. (A) Schematic representation of the puromycin incorporation assay used to quantify translation output at the single-cell level. (B) Quantitative Western blotting to detect the amounts of newly incorporated puromycin in lysates of RACK1-expressing cells. (C) Representative FACS analyses for puromycin incorporation correlate the translational rate and the expression of wild-type or mutant RACK1. (D) Comparison of the amounts of the TMR-positive, puromycin-positive populations from the experiment described in the text. Data are means and standard deviations of results from three independent experiments. Statistical significance (***, P < 0.001) was determined by the t test.