FIG 8.

Regulation of XBP-1s expression. Cells maintained in serum and grown to 70% confluence were treated with ABC294640 (25 μM) or SKi (10 μM) for 24 h. In certain cases, cells were treated with scrambled or Degs1 siRNA (final concentration of 100 nM) for 48 h or with MG132 (10 μM) for 30 min prior to treatment with SKi or ABC294640. A Western blot probed with anti-XBP-1s antibody shows the effect of Degs1 siRNA treatment on the induction of XBP-1s by MG132 in the presence and absence of SKi or ABC294640. Blots were reprobed for GAPDH using anti-GAPDH antibody to ensure comparable protein loading. Results are representative of data from at least 3 independent experiments. Lanes C, control.