rFVIIIFc-treated macrophages exhibit a characteristic metabolic signature, resembling the regulatory phenotype. Metabolite screening was performed on lysates of macrophages (n = 4) treated with IgG1, rFVIII, or rFVIIIFc for 6 hours or 24 hours. (A) Of a total of 578 identified metabolites, 144 were changed significantly between the different treatments. Selected individual metabolites are shown: glycolysis and related metabolism pathways (B), long chain fatty acids (C), and oxidative phosphorylation (D). Mean ± standard deviation; *P ≤ .05. UDP, uracil-diphosphate.