Figure 2.

Co-occurrence of obesity causes pathophysiological changes in an asthma model. (A) Airway hyper-responsiveness was measured as respiratory resistance 24 hours after the final ovalbumin (OVA) challenge. (B) Effect of an high-fat diet (HFD) on cell counts in the bronchoalveolar lavage fluid (BALF). Mice were sacrificed 24 hours after the final OVA challenge, and BALF cells were isolated. (C) Paraffin-embedded lung tissue sections were stained with H&E and periodic acid-Schiff (PAS) (×200). Results are expressed as mean ± SEM (n = 5 to 7 per group). CON, control; Br, bronchus; Bm, basement membrane; Eo, eosinophil; Ep, epithelium; Bl, blood vessel. ^ap < 0.01, ^bp < 0.001 vs. control group, and ^cp < 0.01, ^dp < 0.001 vs. OVA group.