. 2018 Oct 24;2018:8387218. doi: 10.1155/2018/8387218

Table 1.

Primers employed for DEC detection.

Gene	Primer	Sequence 5´-3´	Amplicon Size (bp)	Annealing temperature (°C)	Reference
eae	EAE 1	GAGAATGAAATAGAAGTCGT	775	55	[18]
eae	EAE 2	GCGGTATCTTTCGCGTAATCGCC	775	55	[18]

bfp	EP1	AATGGTGCTTGCGCTTGCTGC	324	55	[19]
bfp	EP2	GCCGCTTTATCCAACCTGGTA	324	55	[19]

stx1	VT1-A	GAAGAGTCCGTGGGATTACG	131	55	[20]
stx1	VT1-B	AGCGATGCAGCTATTAATAA	131	55	[20]

stx2	VT2 a	TTAACCACACCCCACCGGGCAGT	348	55	[20]
stx2	VT2 b	GCTCTGGATGCATCTCTGGT	348	55	[20]

ipaH	EI1	GTTCCTTGACCGCCTTTCCGATACCGTC	620	55	[21]
ipaH	EI2	GCCGGTCAGCCACCCTCTGAGAGTAC	620	55	[21]

pCDV 432	EAEC1	CTGGCGAAAGACTGTATCAT	630	60	[16]
pCDV 432	EAEC2	CAATGTATAGAAATCCGCTGTT	630	60	[16]

eltA	LT-A-1	GGCGACAGATTATACCGTGC	332	55	[16]
eltA	LT-A-2	CCGAATTCTGTTATATATGTC	332	55	[16]

estA	STA-1	ATTTTTATTTCTGTATTGTCTTT	147	48	[16]
estA	STA-2	GGATTACAACACAGTTCACAGCAG	147	48	[16]