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. 2018 Sep 10;4(10):FSO345. doi: 10.4155/fsoa-2018-0077

Figure 1. . Relative SSAT-1 gene expression in human tumor cells and human primary tumor and SSAT-1 protein contents in human tumor tissue.

Figure 1. 

The relative SSAT-1 expression levels in (A) cancer cell lines using GAPDH and hPRT1 as housekeeping genes and (B) primary human breast, prostate and lung tumor tissue normalized with GAPDH as measured by qRT-PCR. Data for tissue expression levels are shown as the mean ± SEM of 6–7 different primary tumor tissues. Experiments were performed in triplicate. Representative immunoblots showing 27 kDa SSAT-1 protein levels were quantified by densitometry using tubulin from four different patients for (C) breast tumor tissue, (D) prostate tumor tissue and (E) lung tumor tissue. Normal human bronchial epithelial cells, prostate epithelial cells and mammary epithelial cells were used as controls as described in the methods.

SEM: Standard error of the mean.