Figure 2.

TIMP3 activity in Zymography. (A) Recombinant human MMP9 was run on Gelatin Zymogram. TIMP3v2 (K22S/F34N) was incubated with Developing buffer overnight after cutting gel lanes and transferring into an individual container. After staining and destaining, the gel was quantified using ImageJ software (lower graph). The gel is a representative of n = 3 independent experiments (mean ± SEM). (B) TIMP3v2 (K22S/F34N) inhibition of cardiac tissue MMP activity on the Zymogram from pig cardiac lysates (50 μg/lane) after 4.5 hours post‐MI. Cardiac tissue was homogenized in RIPA buffer with PMSF. After staining and destaining, the gel was quantified using ImageJ software (lower graph). The gel is a representative of n = 3 experiments (mean ± SEM). Graphs are averages of each ImageJ scan. *P < 0.05 (vs control), **P < 0.01 (vs control)