Figure 7.

AnkG Loss Has a Direct Impact on Myocyte Desmoplakin and PKP2 Expression

(A to D) AnkG, desmocollin-2 (DSC2), desmoplakin (DSP), and plakophilin-2 (PKP2) expression in control and AnkG cKO hearts at baseline and following 2 weeks TAC (n = 3; p < 0.05). (E to H) PKP2 localization in control and AnkG myocytes at baseline and following 2 weeks TAC. Yellow arrows denote disc, whereas white arrowheads denote PKP2 intracellular localization in AnkG cKO myocytes. Scale bar = 20 μm. (I to L) αII-spectrin (low molecular weight form), β-catenin, N-cadherin, and α-tubulin expression in control and AnkG cKO hearts at baseline and following 2 weeks TAC (n = 3; p < 0.05). In multiple cases, GAPDH is the same because blots were either cut or reprobed using unique antibodies for the primary probe. Abbreviations as in Figures 2 and 3