Table 2.
Primers Used in Site-Directed Mutagenesis and PCR
| Primer Name | Primer Sequence (5′ to 3′) |
|---|---|
| Site-Directed Mutagenesis | |
| hDYSF DSC-B GFP REV | CTACTACTTGTACAGCTCGTCCATGC |
| hDYSF DSC-B GFP FWD | GGCCGCTCGAGTCTAGAGGG |
| hDYSF DSC-B Ex49 FWD | GTTTTTCCTGCGTTGTATTATC |
| hDYSF DSC-B Ex45 REV | ACACAGTAGGTCTGTGGG |
| hDYSF DSC-B Ex22 FWD | GAAGCTGGCCTGGAGCAG |
| hDYSF DSC-B Ex18 REV | CTTGCCTGTGTTGAGCTCTG |
| hDYSF DSC-B Ex19 REV | CTCCACCCGGAGGATGTC |
| hDYSF DSC-B Ex28 FWD | AACGGGGAGAAGGTGCTT |
| hDYSF DSC-B Ex25 REV | GGTTTCAGCAAAGACAGACAG |
| hDYSF DSC-B Ex30 FWD | CACAGGCAGGCGGAGGCG |
| hDYSF DSC-B Ex27 REV | CACATCGGTGTAGTTGTCACTCATGTAGATCC |
| RT-PCR | |
| hDYSF Ex26 FWD | CGAGACTAAGTTGGCCCTTG |
| hDYSF Ex30 REV | GGCATCTGTCTTGCGGTACT |