Fig. 2.

Overexpression of ROXY9 reduces chlorophyll content while overexpression of ROXY15 increases chlorophyll content. Images of shoots of three-week-old plants grown in the soil. a Wild-type plants and two independent transgenic plants overexpressing ROXY9 or ROXY15 (ox) were grown for three weeks in the soil and their leaf chlorophyll contents were measured as described in the Method section. b Total chlorophyll content of plants shown in (a). Fully developed mature leaves were used for the measurement of chlorophyll content. The total chlorophyll content was expressed as micrograms per milligram leaf fresh weight. Values are mean ± SE from two leaves per plant (n = 10 plants). Results from one of two independent experiments are shown. c Total chlorophyll content of wild type Col-0 plants deprived of nitrate. Ten-day-old seedlings grown on complete nutrient media were treated with either 4 mM nitrate (+N) or no nitrate (-N) for 4 days. Values are mean ± SE from 5 shoots per replicate (n = 5 replicates). Results from one of two independent experiments are shown. Different letters above the bars indicate values that are significantly different (P < 0.05; t test)