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. 2018 Nov 13;18:281. doi: 10.1186/s12870-018-1512-1

Fig. 3.

Fig. 3

Overexpression of ROXY9 stimulates root hair elongation while overexpression of ROXY15 inhibits root hair elongation. (a, b) Light microscopy images showing root hairs of wild-type Col-0 seedlings and two independent transgenic seedlings overexpressing ROXY9 or ROXY15 (ox). Roots from four-day-old seedlings grown on nitrate-sufficient medium were used for the measurement of the root hair length. Bar = 0.5 mm. b Quantified data showing the root hair length of wild-type Col-0 seedlings and two independent transgenic seedlings overexpressing ROXY9 or ROXY15 (n = 10 seedlings, means ± SE). c Root hair length of wild type Col-0 seedlings grown under nitrate-sufficient (+N) and nitrate-deficient (-N) conditions (n = 10 seedlings, means ± SE). Four-day-old roots treated with either 4 mM nitrate or no nitrate for 24 h were used for the measurement of root hair length. Results from one of two independent experiments are shown here. Different letters above the bars indicate values that are significantly different (P < 0.05; t test)