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. 2018 Nov 14;9:311. doi: 10.1186/s13287-018-1045-4

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cultivation and characterization of rat BMSCs. a Morphology of bone marrow cell in passage 1 (a₁, × 100) and passage 3 (a₂, × 100). b Surface markers of BMSCs. The immunophenotype of rat BMSCs was analyzed by flow cytometry. Most cells expressed CD90, CD73, and CD44, but were CD45-negative and CD11b-negative. c Multipotency of BMSCs. Rat BMSCs could differentiate into osteogenic (c₁, × 200), adipogenic (c₂, × 200), and chondrogenic (c₃, × 200) lineages when cultured in differentiation medium