Table 1.

Mobile phase condition of chromatographic separation

Time (min)	Acetonitrile (%)	Methanol (%)	0.5% acetic acid in water (%)
0–30	12–25	0	88–75
30–45	25–23.5	0–11	75–65.5
45–68	23.5–35	11–4	65.5–61
68–85	35	4	61
85–90	35–50	4–0	61–50
90–95	100	0	0
95–110	100	0	0