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. 2018 Nov 13;15:174. doi: 10.1186/s12985-018-1089-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of immunization on CpG combined with HDCV. HDCV, Saline, and HDCV plus CpG at 1.25, 5 and 20 μg, respectively, were used as vaccines. Balb/c mice (n = 10) were immunized i.m. with the above vaccines on D0, 3, 7, 14, and 28. Serum samples were collected on D0, 6, 8, 10, 14, 30, 45 and 90. RFFIT was used to detect RVNA in the sera of mice. The antibody titer was expressed in international units per milliliter (IU/ml), calculated in comparison with the national reference standard serum