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. 2018 Nov 13;15:174. doi: 10.1186/s12985-018-1089-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — RVNA response induced by reduced injections of HDCV-CpG. Balb/c mice (n = 10) were immunized i.m. with HDCV for 5 injections and HDCV plus CpG for 5, 4, 3 and 2 injections. Serum samples were collected on D35. RFFIT was used to detect RVNA in the sera of mice. The antibody titer was expressed in international units per milliliter (IU/ml), calculated in comparison with the national reference standard serum