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. 2018 Oct 17;3:84. Originally published 2018 Jul 17. [Version 2] doi: 10.12688/wellcomeopenres.14493.2

Figure 5. γc is required for IL-15Rα recruitment to the DC IS.

Figure 5.

( A) Representative TIRFM images showing GFP, MHCII (Alexa Fluor 568) and IL-15Rα (Alexa Fluor 633) accumulation at contact interfaces of γc -/- DC, transfected with γc WT-GFP, γc Δc-GFP or ctrl GFP. ( B) Quantification in arbitrary units (AU) of mean IL-15Rα fluorescence at contact interfaces shown in A (N=38-43, mean ± S.E.M). P values, one-way ANOVA. After incubation on bilayers at 37°C in HBS/HSA buffer, cells were fixed and permeabilized to stain for IL-15Rα in PBS buffer. Imaging was performed on a Nikon Ti microscope with a 100x TIRF objective, N.A. 1.49, controlled by Nikon Elements software. Fluorescence images were captured using an Ixon cooled EMCCD camera (512 x 512 pixels, Andor Technology). Mean fluorescence intensity at contact interfaces was quantified from 14 bit images using Metamorph software. Brightness and contrast are adjusted uniformly across image groups for clarity.