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. 2018 Nov 27;3:113. Originally published 2018 Sep 12. [Version 2] doi: 10.12688/wellcomeopenres.14765.2

Figure 2. Maximum Likelihood molecular phylogenetic analysis of Anopheles gambiae complex ITS2 sequences from field-collected mosquitoes.

Figure 2.

The tree with the highest log likelihood (-785.65) is shown. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 42 nucleotide sequences. There were a total of 475 positions in the final dataset. Symbols, colours and codes used for the sequences generated in this study are as follows: W+; individual was Wolbachia positive (solid coloured symbol), W-; individual was Wolbachia negative (empty coloured symbol). DRC, Democratic Republic of the Congo (red); KAL, Kalemie; MIK, Mikalayi; KIN, Kinshasa; KAT, Katana. GHA, Ghana (blue); DOG, Dogo. GUI, Guinea (green); KSK, Kissidougou. MAD, Madagascar (purple); ANT, Antafia. UGA, Uganda (maroon); BUT, Butemba. Different shape coloured symbols are used to differentiate between the different mosquito species. GenBank sequences included (for comparison with sequences generated in this study) are in black with their accession numbers provided. Where GenBank sequence subtrees have been compressed, this is denoted by a solid black diamond symbol. GenBank accession numbers for sequences included in compressed subtrees are: GQ870318.1 and GQ870320.1 for Anopheles bwambae, and GQ870315.1, JN664146.1 and KR014832.1 for Anopheles quadriannulatus.