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. 2018 Nov 27;3:113. Originally published 2018 Sep 12. [Version 2] doi: 10.12688/wellcomeopenres.14765.2

Figure 5. Wolbachia multilocus sequence typing (MLST) phylogenetic analysis of resident Wolbachia strains in An. coluzzii, An. moucheti and An. species A.

Figure 5.

( A) Maximum Likelihood molecular phylogenetic analysis from concatenation of all five MLST gene loci for resident Wolbachia strains from An. coluzzii ( wAnga-Ghana; blue), An. moucheti ( wAnM; green) and An. species A ( wAnsA; red). The tree with the highest log likelihood (-10606.13) is shown and drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 94 nucleotide sequences. There were a total of 2067 positions in the final dataset. Concatenated sequence data from Wolbachia strains downloaded from MLST database for comparison are shown with isolate numbers in brackets (IsoN). Wolbachia strains isolated from mosquito species highlighted in navy blue, bold. Strains isolated from other Dipteran species are shown in navy blue, from Coleoptera in olive green, from Hemiptera in purple, from Hymenoptera in teal blue, from Lepidoptera in maroon and from other, or unknown orders in black. ( B) Maximum Likelihood molecular phylogenetic analysis for coxA gene locus for resident Wolbachia strains from An. coluzzii ( wAnga-Ghana; blue), An. moucheti ( wAnM; green) and An. species A ( wAnsA and wAnsA(2); red). The tree with the highest log likelihood (-1921.11) is shown and drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 84 nucleotide sequences. There were a total of 402 positions in the final dataset. Sequence data for the coxA locus from Wolbachia strains downloaded from MLST database for comparison are shown in black and navy blue with isolate numbers (IsoN) from the MLST database shown in brackets. Wolbachia strains isolated from mosquito species highlighted in navy blue. GenBank sequence for wAnga-Mali coxA shown in maroon with accession number.