Table 2. Novel resident Wolbachia strain WSP typing and multilocus sequence typing (MLST) gene allelic profiles.
Novel allele numbers (in bold) assigned by the Wolbachia MLST database for strains from An. species A ( wAnsA) and An. moucheti ( wAnM) are shown, alongside the novel allelic profile from An. coluzzii ( wAnga-Ghana), comprising exact matches to existing alleles present in the database for each gene locus. (HVR; Hypervariable regions within the wsp sequence.).
| Mosquito species |
Wolbachia
strain |
WSP typing allele numbers | MLST gene allele numbers | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| wsp | HVR1 | HVR2 | HVR3 | HVR4 | gatB | coxA | hcpA | ftsZ | fbpA | ||
| An. species A | wAnsA | 728 | 254 | 288 | 284 | 23 | 279 | 274 | 302 | 240 | 445 |
| An. moucheti | wAnM | - | - | - | - | - | 280 | 275 | 303 | 241 | 446 |
| An. coluzzii | wAnga-Ghana | - | - | - | - | - | 9 | 64 | 3 * | 177 | 4 |
*Alternative degenerate primers (set 3) were used to generate sequence from another An. coluzzii individual from the same location to complete the full allelic profile.