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. 2018 Nov 7;9:2652. doi: 10.3389/fmicb.2018.02652

Table 3.

Cereulide concentrations (ng/g) in organs and tissue of pigs orally challenged with single doses of 30 and 150 μg cereulide kg-1 body weight and daily doses of 10 μg cereulide kg-1 body weight for 7 days.

Single dose (acute trial) Daily dose (chronic trial)
30 μg kg-1
150 μg kg-1
10 μg kg-1
Range Average Range Average Range Average

Brain 0.15–1.41 0.73 ± 0.49
Heart
Kidney 0–0.43 0.21 ± 0.21 0.14–0.37 0.25 ± 0.12
Liver 0.25–0.41 0.37 ± 0.07 0.06–0.24 0.14 ± 0.08
Spleen 1.15–5.29 2.26 ± 1.75 0.10–0.21 0.15 ± 0.06
Lung n.d.
Lymph nodes 0–4.50 2.82 ± 1.71 0.01–0.14 0.07 ± 0.07
Large intestine 0–0.13 0.07 ± 0.06 7.14–45.75 30.50 ± 14.26 1.16–5.05 3.11 ± 1.95
Small intestine 0.34–2.49 1.34 ± 0.88
Stomach 0.11–7.54 2.00 ± 3.20 n.d.
Fat abdominal 2.17–12.02 7.88 ± 4.17 n.d.
Fat subcutan. 0.35–1.37 0.74 ± 0.51 0–0.08 0.04 ± 0.04
Muscle 0–0.76 0.39 ± 0.32 0–2.23 1.12 ± 1.12
Content large intestine 0–0.09 0.07 ± 0.03 5.84–140.99 67.88 ± 50.80 2.04–6.78 4.41 ± 2.37
Content small intestine n.d. 0–1.06 0.68 ± 0.53 n.d.

Animals were necropsied 48 h after dietary exposure to cereulide in the acute trials and 7 days after start of the experiment in the chronic trail. Concentration of cereulide was determined in organs and tissues by SIDA-LC-MS, using isotope labeled 13C6-cereulide as internal standard (Bauer et al., 2010).