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. 2018 Nov 9;9:310. doi: 10.1186/s13287-018-1049-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Representative figure of migration assays of MSCs, in transwell dishes, in different conditions placed in the lower chambers: a MSCs not labeled [control], b conditioned medium supplemented with specific neutralized antibodies (anti-MCP-1 and anti-SDF-1), c conditioned medium supplemented with specific neutralized antibodies (anti-MCP-1), d conditioned medium supplemented with specific neutralized antibodies (anti-SDF-1), e CD133⁺ cell culture supernatants (TBSCM), f chemokines MCP-1 and SDF-1, g graph summarized of mean number of migrated MSCs in relation to different conditions, h RT-PCR analysis of MCP-1/CCL2 mRNA levels expressed in CD133⁺ GBM cells (n = 5; GBM1 GBM2 GBM3 GBM4 GBM5). i RT-PCR analysis of SDF-1/CXCL12 mRNA levels expressed in CD133⁺ GBM cells (n = 5; GBM1 GBM2 GBM3 GBM4 GBM5). L50: Ladder 50 bp; LM low mass ladder; R positive control (human reference total RNA Clontech)