Fig. 5.

Implantation of 1 × 10⁴ CD133⁺ GBM cells labeled Qdots (705 nm), after the establishment of the GBM (28 days), was made the infusion in caudal vein 1 × 10⁴ MSCs (MION-Rh); the development of tumor was followed for 20 days. a MSCs labeled MION-Rh and CD133⁺ GBM cells labeled Qdot 705 nm using combined fluorescence and X-ray detection. b CD133⁺ GBM cells labeled Qdot 705 nm and visualized by fluorescence detection. c MSCs labeled MION-Rh and visualized by fluorescence detection. d–h, i–l MRI (T₂*-weighted images) of animal brain monitoring of the process of migration of MSCs, which were able to cross the blood-brain barrier of the animal and migrated to the tumor region, promoting GBM cell proliferation. l MRI (T₂*-weighted images) of animal brain without stereotaxic implantation of cells (control group). Red circle showed migration assays of MSCs and green circle evidenced tumor propagation. m IHC analysis for Prussian blue staining of the MSCs labeled with MION-Rh. n, r, s, t Hematoxylin and eosin staining. u IHC analysis for GFAP. o IHC analysis for Ki67; v IHC analysis for p53; p IHC analysis for CD44 staining of the MSCs; q IHC analysis for CD73 staining of the MSCs; w, x IHC analysis for CD63 staining of the MSCs-derived exosomes; y–z′ IHC analysis for CD9 staining of the MSCs-derived exosomes. These images are representative of all collected MSCs and GBM samples