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. 2018 Nov 10;13:9. doi: 10.1186/s13008-018-0042-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression and localization of Kif18a in mouse oocytes. a Oocytes at different maturation stages were examined by Western blotting. b Oocytes from GV to MII stages were stained with anti-Kif18a antibody (green) and counterstained with DAPI for DNA visualization (red). After GVBD, Kif18a accumulated around chromosomes. Also, Kif18a localized in the meiotic spindle region at both the MI and MII stages, while it localized in the midbody at the ATI stage. Negative control was stained with secondary antibody without Kif18a. Scale bar: 20 μm